Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae

BACANELLI, GISELE; OLARTE, LARISSA C.; SILVA, MÁRCIO R.; RODRIGUES, RUDIELLE A.; CARNEIRO, PAULO A.M.; KANEENE, JOHN B.; PASQUATTI, TAYNARA N.; TAKATANI, HARUO; ZUMÁRRAGA, MARTIN J.; ETGES, RODRIGO N.; ARAÚJO, FLÁBIO R.; VERBISCK, NEWTON V.

JOURNAL OF VETERINARY MEDICAL SCIENCE

JAPAN SOC VET SCI

Lugar: Tokio; Año: 2019

0916-7250

In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry was used to identify Mycobacterium bovisfrom cattle and buffalo tissue isolates from the North and South regions of Brazil,grown in solid medium and previously identified by Polymerase Chain Reaction (PCR)based on Region of Difference 4 (RD4), sequencing and spoligotyping. For thispurpose, the protein extraction protocol and the mass spectra reference database wereoptimized for the identification of 80 clinical isolates of mycobacteria. As a result ofthis optimization, it was possible to identify and differentiate M. bovis from othermembers of the Mycobacterium tuberculosis complex with 100% specificity, 90.91%sensitivity and 91.25% reliability. MALDI-TOF MS methodology described hereinprovides successful identification of M. bovis within bovine/bubaline clinical samples,demonstrating its usefulness for bovine tuberculosis diagnosis in the future.