Comparative Analysis Of Alkaline/Neutral Invertases Among Plants.

LECHNER, LEANDRA; BATTAGLIA, MARINA; MARTIN, MARIA VICTORIA; MARTINEZ-NOEL, GISELLE; SALERNO, GRACIELA

MAR DEL PLATA

Congreso; Sociedad Argentina de Investigacion en Bioquimica y Biologia Molecular; 2015

SAIB

Sucrose (Suc) plays a central role in plant growth and development, and in response to environmental stresses.Alkaline/Neutral-Invertases (A/N-Inv) (localized in the cytosol, mitochondria and/or in plastids) hydrolyze Suc tohexoses for maintenance functions. Recently significant roles in root morphogenesis, germination, photosyntheticapparatus biosynthesis, abiotic and biotic stress tolerance have been revealed. A/N-Inv multigene families have beenreported in A. thaliana (9 genes), O. sativa (8), P. trichocharpa (16), V. vinifera (9), L. japonicus (7) and M.esculenta (11). In the present study we identified the A/N-Inv family in the wheat genome, analyzed its structure,phylogeny and expression patterns, which were compared with other plants. Similarly to rice, we identified 4 and 3putative genes coding for cytosolic (Ta-NIN5 to 8) and organelle isoforms (Ta-NIN1 to 4), respectively, whosesequences were categorized into the two major clades described. Expression analyses showed that genes coding forcytosolic isozymes expressed in roots and leaves, and were differentially regulated in response to cold and droughtstress. We conclude that organellar wheat A/N-Invs are conserved and common to distantly related plants, and someof the cytosolic isoforms are induced in response to environmental stresses. Supported by CONICET, ANPCyT(PICT 1288), UNMdP and FIBA.