INVOLVEMENT OF HISTAMINE H4 RECEPTOR IN THE HISTAMINE-INDUCED MODULATION OF TUMOR GROWTH AND ANTITUMOR IMMUNITY IN 4T1 TRIPLE NEGATIVE BREAST CANCER

STERLE, HELENA A.; NICOUD, MELISA B; LAMAS, DJMARTINEL; JUAN CARLOS PERAZZO; CREMASCHI, GRACIELA A.; MEDINA, VANINA A

Mar del Plata

Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2016

Sociedad Argentina de Investigación Clínica

Histamine is known to regulate cellular immune responses, affectingmaturation and activity of lymphocytes and myeloid-derivedsuppressor cells (MDSC). However, its role in the regulation ofantitumor immunity has not been well established. The aim of thepresent work was to investigate the influence of the lack of histamineH4 receptor (H4R) on the tumorigenic capacity, tumor celldissemination and the antitumor immunity in response to histaminein a triple negative breast cancer (TNBC) model. For that purpose,we evaluated tumor growth parameters and the composition ofsplenic and lymph node immune subsets in syngeneic H4R knockout(H4R KO) and wild-type (WT) mice, inoculated orthotopicallywith 4T1 murine TNBC cells. Percentages of CD4+, CD8+, CD19+,CD3+, CD11b+Gr1+ and NK1.1+ cells were quantified in spleensand lymph nodes by flow cytometry. In vitro results indicate thathistamine (0.1-10 μM) reduced the cell proliferation levels of the4T1 TNBC, evaluated by the clonogenic assay (p