GASTRIC EMPTYING RELATED EFFECT OF Capparis atamisquea LEAF EXTRACTS. In vivo ASSAYS.

FÉLIX FACUNDO TABOADA; NATALIA HABIB; SUSANA GENTA

Jornada; "XXXIV Jornadas Científicas de la Asociación de Biología de Tucumán?; 2017

Atamisqui (Capparis atamisquea Kuntze) is an autochthonous plant widely distributed in Chile and northwest Argentina, traditional medicine attributes digestive property. The delay of the gastric emptying has its origin in a motor disorder, anthric stenosis, or diabetic neuropathy?s gastroparesis. The search for more effective and safe prokinetic drugs is a current challenge. The aim of the present study was to evaluate the effect on gastric emptying of C. atamisquea leaves 5% infusion (I) and 10% hydroalcoholic extract in 70 º ethanol (EHA). Also, qualitative and quantitative phytochemical studies of the extracts were carried out. A gastric emptying test was performed on male Wistar rats using 0.05% w / v Phenol Red marker. (n = 6 animals/group): 1-negative control, 2 and 3: positive controls (Metoclopramide 3 mg / kg orally and Cinitapride, 1 mg / kg orally) 4 and 5: treated respectively with I and EHA (150 mg / kg orally). To study the involvement of the cholinergic pathway in the observed effect an in vivo atropine blockade (3mg / kg subcutaneous route) was performed. The extracts analyzed accelerated gastric emptying significantly p≤0.05 in comparison to the negative control, in addition this effect was blocked by atropine pretreatment. Therefore, these results suggest that Atamisqui leaf extracts have a gastric prokinetic effect and the cholinergic route would be involved. Additional studies will be needed to determine the active principle (s) and to know better the underlying mechanisms.