Evaluation of different total Leishmania amazonensis antigens for the development of a first-generation vaccine formulated with a Toll-like receptor-3 agonist to prevent cutaneous leishmaniasis

GERMANÓ, MARÍA JOSÉ; LOZANO, ESTEBAN SEBASTIÁN; SANCHEZ, MARÍA VICTORIA; BRUNA, FLAVIA ALEJANDRA; GARCÍA-BUSTOS, MARÍA FERNANDA; SOSA LOCHEDINO, ARIANNA LOURDES; SALOMÓN, MARÍA CRISTINA; FERNANDES, ANA PAULA; MACKERN-OBERTI, JUAN PABLO; CARGNELUTTI, DIEGO ESTEBAN

MEMóRIAS DO INSTITUTO OSWALDO CRUZ.

FUNDACO OSWALDO CRUZ

Lugar: Rio de Janeiro; Año: 2020 vol. 115

0074-0276

BACKGROUND Unfortunately, no any vaccine against leishmaniasis has been developed for human use. Therefore, a vaccine based on total Leishmania antigens could be a good and economic approach; and there are different methodologies to obtain these antigens. However, it is unknown whether the method to obtain the antigens affects the integrity and immune response caused by them. OBJECTIVES To compare the protein profile and immune response generated by total L. amazonensis antigens (TLA) produced by different methods, as well as to analyze the immune response and protection by a first-generation vaccine formulated with sonicated TLA (sTLA) and polyinosinic:polycytidylic acid [Poly (I:C)]. METHODS TLA were obtained by four different methodologies and their integrity and immune response were evaluated. Finally, sTLA was formulated with Poly (I:C) and their protective immune response was measured. FINDINGS sTLA presented a conserved protein profile and induced a strong immune response. In addition, Poly (I:C) improved the immune response generated by sTLA. Finally, sTLA + Poly (I:C) formulation provided partial protection against L. amazonensis infection. MAIN CONCLUSIONS The protein profile and immune response depend on the methodology used to obtain the antigens. Also, the formulation sTLA + Poly (I:C) provides partial protection against cutaneous leishmaniasis in mice.