Genomics and proteomics of bacteriocin-producing strain Pseudomonas fluorescens SF4c

GODINO, A.; FERNANDEZ, M.; PRÍNCIPE, A.; FISCHER, S.E.

Córdoba

Congreso; 52 Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular.; 2016

Sociedad Argentina de Investigación en Bioquímica y Biología Molecular

Bacteriocins are narrow-spectrum antimicrobial with high potential for application as biocontrol agents. Characterization of new bacteriocins with different action spectra becomes increasingly relevant. In this context, we perform a genome mining study to predict bacteriocin genes in the draft genome of P. fluorescens SF4c. This in silico analysis revealed the presence of a complete tailocin (phage-like bacteriocins) cluster with structural genes for R (contractile) and F (flexible) -type tailocins. Moreover, two genes corresponding to S-type pyocins (soluble proteins) with their cognate immunity proteins were also identified in the SF4c genome. To analyze if the predicted bacteriocins are produced by SF4c a proteomics study was performed. Extracts of supernatants of SF4c cultures induced with mitomicin C were digested with trypsin and analyzed by nano-HPLC coupled to mass spectrometer with Orbitrap technology (Mass Spectrometry Service of CEQUIBIEM-UBA). The spectra were analyzed against SF4c proteome obtained from draft genome. Data analysis showed that all bacteriocin predicted from genome were identified in the SF4c extract, including the 27 structural proteins that form the R and F tailocin structure and the 2 pyocins S with their respective immunity proteins. These results show that SF4c strain is able to produce various bacteriocins with different structures and proprieties.