BECAS
SALASSA Betiana NebaÍ
congresos y reuniones científicas
Título:
Hemin induces autophagy in leukemic erythroblast cell line
Autor/es:
SALASSA B.N., MOOR F., VERGARA A.N., FADER C.M.
Lugar:
Buenos Aires
Reunión:
Encuentro; Reunión Anual SAIB; 2013
Institución organizadora:
SAIB
Resumen:
Autophagy is a normal degradative pathway that involves the sequestration of cytoplasmic components and organelles in a vacuole called autophagosome, which finally fuses with the lysosome to degrade the sequestered material. This process has been associated with several physiologic processes as erythroid maturation. LC3 is a protein present in autophagosomal membrane, therefore is considered a bonafide marker of this structure. Our results indicate that in K562 cells, hemin (erythroid maturation inductor) produced an increased number and enlargement of GFP-LC3 positive vesicles labeled with lysotracker or DQ-BSA compared with others maturation inductors. Moreover, we have demonstrated that hemin induces mitochondria membrane depolarization. We have also shown in erythroblastic leukemic cells co-expressing RFP-LC3 and GFPRab11 (a multivesicular bodies marker) that hemin generates an enlarged vesicles labeled with both markers. Likewise, we have performed a western blot to detect the processing of LC3 protein upon hemin incubation. We have observed in this assay an increased total amount and a higher level of processed LC3. Taken together, our results suggest that hemin could induce mitophagy response in K562 cells, probably allowing an efficient and faster maturation