Evaluation of SRAP markers for mapping of Pisum sativum L.

GUINDÓN, MF; MARTIN, EA; ZAYAS, ALDANA; COINTRY, EL; CRAVERO, VP

CROP BREEDING AND APPLIED BIOTECHNOLOGY

BRAZILIAN SOC PLANT BREEDING

Año: 2016

1518-7853

Linkage maps have become important tools for genetic studies. Withthe aim of evaluating the SRAP (sequence-related amplified polymorphism)technique for linkage mapping in Pisum sativum L., a F2 mapping populationderived from an initial cross between cvs. DDR11 and Zav25 was generated. Atotal of 25 SRAP primer combinations were evaluated in 45 F2 plants and bothparental lines, generating 208 polymorphic bands/markers. The markers wereanalyzed by the chi-square goodness-of-fit test to check the expected Mendeliansegregation ratio. The resulting linkage map consists of 112 genetic markersdistributed in 7 linkage groups (LGs), covering a total of 528.8 cM. The length ofthe LGs ranged from 47.6 to 144.3 cM (mean 75.54 cM), with 9 to 34 markers.The linkage map developed in this study indicates that the SRAP marker systemcould be applied to mapping studies of pea.