Evaluation of the Response of Hyalella curvispina from a River in Norpatagonia Exposed to Caffeine

AGUIAR MARIA BELEN; PARRA MORALES LAURA; MORELL MALENA; LATINI LORENA

Montevideo

Congreso; SETAC Latín América 15th biennial meeting; 2023

bstractThe world population is constantly increasing as well as the development of socioeconomic activities. Thissituation causes an uncontrolled increase of environmental pollutants. Many of these reach water bodiesthrough the discharge of industrial, municipal, and agricultural effluents. Caffeine (CF) is an organiccompound present in consumer beverages and it is used as a psychostimulant, analgesic, andbronchodilator. Moreover, it is considered an efficient pollution indicator of anthropogenic activity due to itswidespread use in the population and its frequently detected, mainly in aquatic environments.Neuquén is the second province with the higher population growth rate in the last 12 years, with an averageof 40 inhabitants/day. This population growth represents a potential pollutant risk in water bodies that crossurbanized areas. The aim of this work was to evaluate and compare the response of laboratory and fieldpopulations Hyalella curvispina from the Neuquén River. Acute toxicity tests were carried out at 24, 48 and72 hours with Hyalella curvispina exposed to CF at different concentrations. For each CF concentrationrange (100-998 mg L-1) at least 4 replicates were performed with their respective controls per experimentalunit. The tests were static and without feeding during their development. Mortality (%M) was recorded whenno movement of the pleopods was observed. Within the range of concentrations tested, only 100 %M wasobtained at 72h. The concentration-response relationship as a function of the log of the concentrationshowed a different behavior than expected. The results showed that it did not follow logarithmic behavior.However, it was observed that laboratory organisms show a higher %M to CF at all exposure times thanfield population, suggesting a greater sensitivity to CF. According to these results biochemical studies willbe carried out. It should be noticed that these studies are in a preliminary stage to evaluate toxicologicalparameters and the risk factor calculation.