Low-cost homemade cocktails for enzymatic conversion of sugarcane and cassava bagasses

DÍAZ GABRIELA VERÓNICA; SAWOSTJANIK AFANASIUK SILVANA SOLEDAD; CONIGLIO ROMINA OLGA; JUAN ERNESTO VELÁZQUEZ; MARÍA DANIELA RODRÍGUEZ; PEDRO DARÍO ZAPATA; LAURA LIDIA VILLALBA; MARÍA ISABEL FONSECA

ENVIRONMENTAL TECHNOLOGY

TAYLOR & FRANCIS LTD

Lugar: Londres; Año: 2022

0959-3330

The agricultural industries generate lignocellulosic wastes that can be modified by fungi togenerate high value-added products. This work aimed to analyze the efficiency and the costeffectiveness of the bioconversion of sugarcane and cassava bagasses using low-costhomemade enzymatic cocktails from Aspergillus niger LBM 134. Both bagasses were pretreatedwith a soft alkaline solution without any loss of polysaccharides. After the hydrolysis, a 28% ofconversion to glucose and 42% to xylose were reached in the hydrolysis of sugarcane bagassewhile an 80% of saccharification yield, in the hydrolysis of cassava bagasse using thehomemade enzymes. Furthermore, a more disorganised surface and no starch granules wereobserved in the sugarcane and cassava bagasses, respectively. The bioethanol yield fromsugarcane and casava bagasses was predicted to be 4.16 mg mL−1 and 2.57 mg mL−1,respectively. A comparison of the cost of the homemade and the commercial enzymes wascarried out. Similar hydrolysis percentages were achieved employing any enzyme; however, itwas 1000–2000 times less expensive using the homemade cocktails than using the commercialenzymes. Therefore, the cost of obtaining glucose from bagasses was most expensive whenapplying the commercial enzymes. Moreover, the hydrolysis of the cassava bagasse was mostefficient with the homemade cocktails. The importance and novelty of this work lie in thesimilar performance and the lower cost of the homemade cocktails from the fungus A. nigerLBM 134 compared with the commercial enzymes on the hydrolysis of the sugarcane andcassava bagasses.