AVR and ECP polimorphisms within representatives of race 0 and 2 isolates of Fulvia Fulva (syn. Cladosporium fulvum) the causative agent leaf mold disease

LUCENTINI, CÉSAR; MEDINA, ROCIO; LOPEZ, SILVINA; REPARAZ, JUAN; GAUNA, JUAN; SAPARRAT, MARIO; BALATTI, PEDRO

Montevideo

Congreso; X Encuentro Latinoamericano y del Caribe de biotecnología agropecuaria y xi simposio REDBIO Argentina.; 2019

REDBIO

Fulvia fulva is a pathogenic fungus that causes leaf mold of greenhouse-growth tomatoes. Inprevious studies, two races (0 y 2) of F. fulva were identified within isolates collected from theproduction areas in Argentina, whose genomes proved to be polymorphic. The purpose of thisstudy is to analyze the population diversity at the avirulence (Avr) genes level using molecularmarkers. Four-teen monosporic cultures were typed by means of ISSR-PCR (Inter-simplesequence repeat) markers. The 7 ISSR-PCR primers selected amplified 175 bands. Amongthem, 54 amplicons were recorder polymorphic (31 %). All the isolates of F. fulva wereclustered in groups at a similarity coefficient of 0.875. The Avr, as well as the extracellularprotein (Ecp) genes of the same 14 isolates, were sequenced, analyzed and compared withavailable sequences at the NCBI. Among the polymorphisms found it can be mentionednucleotide variations (SNPs), transversions and transitions, insertions and deletions. Thelatter included the deletion of a 200 pb part of Ecp5 (CIDEFI326). Avr4E, presented twochanges compared to available sequence (AY546101), where 364cytosine was substituted bytimine and 398cytosine was replacement by timine; in this way it generated two changes from 82Leucine to Phenylalanine and of de 93Tyrosine to Methionine, such changes might make Cf4E unrecognizable by the corresponding R gene. Here we found that ecp genes are highlyvariable though we still need to understand the biological role of such changes. Among theisolates, two representatives of race 2, CIDEFI329 and CIDEFI300 were the most polymorphicones, we are still trying to understand the biological significance of this is still under study.