FREQUENCY OF HUMAN PLATELET ANTIGEN (HPAs) POLYMORPHISMS IN DONORS FROM DIFFERENT POPULATION GROUPS OF ROSARIO CITY

MATTALONI, STELLA MARIS; MIGONI, ARIANA; ENSINCK, MARÍA ALEJANDRA; LUJAN BRAJOVICH, MELINA; TRUCCO BOGGIONE, CAROLINA; MUFARREGE, NICOLÁS; PRINCIPI, CINTIA; GARCIA BORRAS, SILVIA; BIONDI, CLAUDIA; COTORRUELO, CARLOS

Rosario

Congreso; XX Congreso y XXXVIII Renión Anual de la Sociedad de Biología de Rosario; 2018

Sociedad de Biología de Rosario

Diversity of HPAs is based on a single base pair substitution in the platelet glycoprotein complexes genes. HPA are involved in foetal and neonatal alloimmune thrombocytopenia, post-transfusion purpura and platelet transfusion refractoriness. The frequencies of HPAs vary between different populations. Considering that our current population is the result of a complex process of hybridization between Caucasians, Native Americans and Africans and that the admixture degree varies among different social groups, we consider critical to evaluate the distribution of platelet phenotypes in our population. The aim of this study was to investigate the genotype and allele frequencies of the HPA-1, HPA-2, HPA-3, HPA-5 and HPA-15 systems in two different groups: donors from a public hospital [G1] and donors from a private laboratory [G2]. Blood samples were obtained from 264 individuals from G1 and 258 from G2. HPA typing was performed by molecular strategies based on PCR-SSP and PCR-RFLP. HPA-1 genotype frequencies (GF) for G1 were: 1a/1a=0.83, 1a/1b=0.17, 1b/1b=0.00 while for G2 were 1a/1a=0.70, 1a/1b=0.28, 1b/1b=0.02. The allele frequencies (AF) found for G1 were: HPA-1a=0.92, HPA-1b=0.08 while for G2 were HPA-1a=0.84, HPA-1b=0.16. HPA-2 GF for G1 were: 2a/2a=0.73, 2a/2b=0.26, 2b/2b=0.01 while for G2 were: 2a/2a=0.85, 2a/2b=0.13, 2a/2b=0.02. The AF found for G1 were: HPA-2a=0.86, HPA-2b=0.14 while for G2 were HPA-2a=0.91, HPA-2b=0.09. HPA-3 GF for G1 were: 3a/3a=0.38, 3a/3b=0.47, 3b/3b=0.15 while for G2 were: 3a/3a=0.44, 3a/3b=0.53, 3b/3b=0.03. The AF found for G1 were: HPA-3a=0.62, HPA-3b=0.38 while for G2 was HPA-3a=0.71, HPA-3b=0.30. HPA-5 GF for G1 were: 5a/5a=0.81, 5a/5b=0.19, 5b/5b=0.00 while for G2 were 5a/5a=0.30, 5a/5b=0.69, 5b/5b=0.01. The AF found for G1 were: HPA-5a=0.90, HPA-5b=0.10 while for G2 were HPA-5a=0.65, HPA-5b=0.35. HPA-15 GF for G1 were: 15a/15a=0.34, 15a/15b=0.32, 15b/15b=0.34 while for G2 were 15a/15a=0.26, 15a/15b=0.29, 15b/15b=0.45. The AF found for G1 were: HPA-15a=0.50, HPA-15b=0.50 while for G2 were HPA-15a=0.41, HPA-15b=0.59. According to chi-square test, statistically significant differences were found for the HPA genotype distribution between both groups (HPA-1: p