Analysis of erythrocyte membrane-bound autologous IgG in adult and newborn blood samples

MUFARREGE, NICOLÁS; ENSINCK, MARÍA ALEJANDRA; RACCA, LILIANA; TRUCCO BOGGIONE, CAROLINA; LUJAN BRAJOVICH, MELINA; MATTALONI, STELLA MARIS; GARCIA BORRAS, SILVIA; BIONDI, CLAUDIA; COTORRUELO, CARLOS

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Congreso; LXI Reunión Científica Anual de la Sociedad de Investigación Clínica. LXIV Reunión Anual de la Sociedad Argentina de Inmunología. XLVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental.; 2016

Sociedad Argentina de Inmunología

While adult red blood cells (RBCs) survive in circulation for 120 days, newborn RBCs survive only 60-80 days. During ageing, senescent (Se) RBCs expose removal markers, as the putative senescence antigen, that account for their selective recognition by macrophages and clearance from circulation. There is evidence that adult RBC ageing leads to the binding of autologous IgG followed by recognition and phagocytosis. However, the senescence process in newborn RBCs is not well characterized. The purpose of this work was to investigate erythrocyte membrane-bound autologous IgG in SeRBCs and Young (Y) RBCs obtained from adult and cord blood samples. Direct fluorescent staining was done on adult (n=20) and cord blood (n=17) erythrocyte suspensions using an anti-human IgG labeled with Alexa 488. SeRBC and YRBC populations were separated by flow cytometry using forward scatter and side scatter parameters. The percentage of IgG positive RBCs was measured in these populations. The average percentage of IgG positive RBCs for adult samples was: YRBCs=0.4±0.25 vs SeRBCs=2.3±1.40 while for the cord ones was YRBCs=0.5±0.12 vs SeRBCs=5.2±2.86. A significant increase in membrane-bound IgG in SeRBC populations of both adult and cord RBCs (p