Influence of the RH phenotype on the D antigen expression

LUJÁN BRAJOVICH, MELINA ELIANA; TRUCCO BOGGIONE, CAROLINA; MATTALONI, STELLA MARIS; ENSINCK, MARÍA ALEJANDRA; RACCA, LILIANA; BIONDI, CLAUDIA; COTORRUELO, CARLOS

Los cocos, Córdoba

Congreso; LXI Reunión Anual de la Sociedad Argentina de Inmunología; 2013

Sociedad Argentina de Inmunología

Alloimmunization against the D antigen depends on level of expression of the RhD polypeptide in the red blood cell membrane. The aim of this work was to analyze the D antigen expression in RhD positive samples with different Rh complete phenotype by flow cytometry. The Rh phenotype on erythrocyte suspensions was determined by hemagglutination using monoclonal antibodies anti-D, C, c, E and e. RhD positive samples and controls (RhD negative) were incubated with monoclonal IgG anti-D and then with anti-human IgG labeled with Alexa 488. One hundred thousand events were acquired and data were analyzed with the FACSDiva software. Statistical analysis showed that the median and interquartile range for the variable median of the fluorescence intensity associated with the D antigen expression were: samples with Ccee phenotype (n=9) 10816,0 ± 3430,0; CCee (n=8) 14731,0 ± 11354,5; ccEe (n=6) 16906,5 ± 8546,0; CcEe (n=9) 20508,0 ± 13619,0 and ccEE (n=2) 28267,0 ± 686,0. The value obtained was significantly higher in ccEE samples when compared with Ccee samples (p