Clostridioides difficile: Characterization of the circulating toxinotypes in an Argentinean public hospital

CRIVARO, ANDREA; CARASI, PAULA; SALTO, ILEANA; HUGO, AYELEN; SOLDAVINI PELICHOTTI, CECILIA; BENGOA, ANA AGUSTINA; FRAGOMENO, MELISA ; SERRADEL, MARIA; MINNAARD, JESSICA; ROLNY, IVANNA; ALUL, EDUARDO; ARREGUI, LEANDRO; FABRA MARTINEZ, MACARENA; MORENO VALERO, OSCAR ; FACENTE, ANDREA; MAGARIÑOS, FRANCISCO; JEWTUCHOWICZ, VIRGINIA; PEREZ, PABLO ; TREJO, FERNANDO

REVISTA ARGENTINA DE MICROBIOLOGíA

ASOCIACION ARGENTINA MICROBIOLOGIA

Lugar: Buenos Aires; Año: 2022

0325-7541

Clostridioides difficile is a spore-forming anaerobe microorganism associated tonosocomial diarrhea. Its virulence is mainly associated with TcdA and TcdB toxins, encoded bytheir respective tcdA and tcdB genes. These genes are part of the pathogenicity locus (PaLoc).Our aim was to characterize relevant C. difficile toxinotypes circulating in the hospital set-ting. The tcdA and tcdB genes were amplified and digested with different restriction enzymes:EcoRI for tcdA; HincII and AccI for tcdB. In addition, the presence of the cdtB (binary toxin)gene, TcdA and TcdB toxins by dot blot and the cytotoxic effect of culture supernatants on Verocells, were evaluated. Altogether, these studies revealed three different circulating toxinotypesaccording to Rupnik?s classification: 0, I and VIII, being the latter the most prevalent one. Eventhough more studies are certainly necessary (e.g. sequencing analysis), it is worth noting thatthe occurrence of toxinotype I could be related to the introduction of bacteria from differentgeographical origins.