Characterization of the primary metabolome during the long-term response to NaHCO3-derived alkalinity in Lotus japonicus ecotypes Gifu B-129 and MiyakojimaMG-20

BORDENAVE C.; ROCCO R.; BABUIN M.F.; CAMPESTRE M.P.; ESCARAY F.J.; GARRIZ A.; ANTONELLI C.J.; CARRASCO SORLI, P.; MENENDEZ A.B.; RUIZ O.A.

Congreso; 11th International Plant Molecular Biology Congress - 2015; 2015

The present study was aimed at analyzing alkalinity-induced changes in the primary leaf metabolome of the L. japonicus ecotypes Gifu B-129 and Miyakojima MG-20.The two ecotypes were evaluated under control (without salt) and alkaline (10 mM NaHCO3) conditions. Gas Chromatography-Mass Spectrometry (GC-MS) analysis ofshoot extracts from both ecotypes was performed, along with measurements of growth, phosphorus and available iron concentrations.Alkalinity induced a higher detrimental effect in terms of chlorosis and survival inalkalinized Gifu B-129 plants, with respect to MG-20 ones. The analysis yielded 41different, quantificable metabolites, including aromatic organic acids (AOA), amino acids (AA), sugars and polyols. We have found some genotype-specific metabolites. At the same time, some metabolites were differentially affected by alkalinity in each genotype.We can conclude that the higher tolerance of MG-20 plants, compared with Gifu B-129 could be related, at least in part, to the differential accumulation of citric, citramalic, lactic and phosphoric acids, and other organic metabolites.