Characterization of the primary metabolome during the longterm response to NaHCO3-derived alkalinity in Lotus japonicus ecotypes Gifu B-129 and Miyakojima MG-20

CESAR D. BORDENAVE; RUBÉN ROCCO; MARÍA FLORENCIA BABUIN; MARÍA PAULA CAMPESTRE; FRANCISCO J. ESCARAY; ANDRÉS GÁRRIZ; CRISTIAN ANTONELLI; PEDRO CARRASCO; OSCAR A. RUIZ; ANA B. MENENDEZ

ACTA PHYSIOLOGIAE PLANTARUM

SPRINGER HEIDELBERG

Lugar: HEIDELBERG; Año: 2017 vol. 39

0137-5881

Abstract This study compares the response of two ecotypesof the model species Lotus japonicas, MG-20 andGifu-B-129, to soil alkalinity, in terms of plant survival andchanges in global primary metabolome profiles. After54 days of treatment with 30 mM NaHCO3, a higher survivalwas registered in MG-20, with respect to Gifu-B-129plants. Gas chromatography?mass spectrometry (GC?MS)analysis of shoot extracts from both ecotypes yielded 123different analytes, 62 of which were identified, includingorganic acids (OA), amino acids (AA), sugars and polyols.Glycolysis, TCA cycle and amino acids metabolism pathwayswere differently affected by alkalinity according tothe ecotype. The lower tolerance of Gifu B-129 plants to10 mM NaHCO3, compared with MG-20 ones could berelated, at least partially, to the differential accumulation ofphosphoric, lactic, threonic, succinic and p-coumaric acids,as well as b-alanine and valine.