INVESTIGADORES
GONZALEZ SCHAIN Nahuel Damian
artículos
Título:
A mitochondrial dysfunction induces the expression of nuclear-encoded complex I genes in engineered male sterile Arabidopsis thaliana
Autor/es:
GÓMEZ-CASATI DF; BUSI MV; GONZALEZ SCHAIN N; MOURAS A; ZABALETA EJ; ARAYA A
Revista:
FEBS LETTERS
Editorial:
ELSEVIER SCIENCE BV
Referencias:
Lugar: Amsterdam; Año: 2002 vol. 532 p. 70 - 74
ISSN:
0014-5793
Resumen:
To study the effect of a mitochondrial dysfunction induced by the
expression of the unedited form of the subunit 9 of ATP synthase gene (u-atp9) in Arabidopsis, we constructed transgenic plants expressing u-atp9
under the control of three different promoters: CaMV 35S, apetala 3 and
A9. The size and shape of transgenic plants bearing the apetala3::u-atp9 and A9::u-atp9 genes looked normal while the 35S::u-atp9 transformed plants showed a dwarf morphology. All u-atp9
expressing plants, independent of the promoter used, exhibited a male
sterile phenotype. Molecular analysis of male sterile plants revealed
the induction of the mitochondrial nuclear complex I (nCI) genes, psst, tyky and nadh binding protein (nadhbp), associated with a mitochondrial dysfunction. These results support the hypothesis that the expression of u-atp9 can induce male sterility and reveal that the apetala3::u-atp9 and A9::u-atp9 plants induced the sterile phenotype without affecting the vegetative development of Arabidopsis
plants. Moreover, male sterile plants produced by this procedure are an
interesting model to study the global changes generated by an
engineered mitochondrial dysfunction at the transcriptome and proteome
levels in Arabidopsis plants