Sequence Analysis of the 5.8S Ribosomal DNA and Internal Transcribed Spacers (ITS1 and ITS2) from five species of the Oxalis tuberosa alliance

DANIELA TOSTO; ESTEBAN HOPP

DNA Sequence, The journal of sequencing and mapping

Taylor and Francis group

Lugar: Nueva York; Año: 1996 vol. 6 p. 361 - 364

1042-5179

The internal transcribed spacer region (ITS1 and ITS21 of the 18S-25S nuclear ribosomal DNA sequence and the intervening 5.8sregion from five species of the genus Oxalis was amplified by polymerase chain reaction and subjected to direct DN A sequencing. On the basis of cytogenetic studies some species of this genus were postulated to be related by the number of chromosomes. Sequence homologies in the ITSI, 5.8s and ITS2 among species are in good agreement with previous relationships established on the basis of chromosome numbers. We also indentified a highly conserved sequence of six bp in the ITSI, reported to be present in a wide range of flowering plants, but not in the Oxalidaceaefamily to which the genus Oxalis belongs to.