THE PHTHALATE DEHP DOWNREGULATES THE PITUITARY ERα EXPRESSION AND IMPACTS ON LACTOTROPH AND GONADOTROPH PROLIFERATION

PÉREZ, PABLO A.; JONATHAN TOLEDO; ANA L. DE PAUL; ALICIA I. TORRES; GUTIERREZ, SILVINA

Mar del Plata

Congreso; Reunion Anual de la Sociedad Argentina de Investigación Clínica; 2019

Sociedad Argentina de Investigación clínica

The normal functioning of the pituitary gland is the result of a balanced mechanism capable of rhythmically control the proliferation and hormonal secretion of different endocrine cell populations in response to endogenous factors such as Estrogen (E2) acting through estrogen receptors (ER). It has been shown that exogenous agents as phthalates, substances used for plastics manufacture, might act as endocrine disruptors in several tissues, simulating E2 effects. The purpose of this study was to analyze the Di-2-ethylhexyl phthalate (DEHP) effect on pituitary ERa expression and its impact on cell proliferation.To carry out this objective we used primary pituitary cultures from female rats which were stimulated with DEHP, E2 or DEHP+E2 for 72h. The lactotroph and gonadotroph proliferation was quantified by double immunostaining (ki67+PRL or Ki67+bLH). The percentages of intracellular ERa (ERa) and membrane ERa (mERa) positive cells were quantified by flow cytometry (FC). For in vivo model female rats prenatally treated with DEHP were maintained for 21days after birth, the pituitary glands were used for ERa quantification by FC. ANOVA Fisher (P