ESTATIONAL AND LACTATIONAL EXPOSURE TO DEHP ALTERS PITUITARY ANDROGEN RECEPTOR EXPRESSION AND GONADOTROPH CELL GROWTH

PABLO A. PÉREZ; JONATHAN TOLEDO; QUINTAR, AMADO; ANA L. DE PAUL; GUTIERREZ, SILVINA

Mar del Plata

Congreso; Reunion anual de Sociedad Argentina de Investigación Clínica; 2022

Sociedad Argentina de Investigación Clínica

Di-(2-ethylhexyl)phthalate (DEHP), an endocrine disruptor used in a variety of commercial products, crosses the plac ental barrier,causing reproductive alterations. The impact on pituitary gland, and in particular, the molecular mechanisms involved are poorly known.We focused on the DEHP effects on pituitary androgen receptor (AR) expression in male and female rats, and explored their influence on gonadotroph cell growth. For in vivo Model,pregnant rats were assigned to 2 groups: DEHP (200 µg/kg body weight/day) and control (corn oil), administrated by gavage until weaning. The offspring were euthanized atpostnatal day (PND) 21 (prepubertal) or PND75 (adult) and the pituitary glands were used in this study.For invitro model, primary pituitary cell cultures were stimulated for 72h with DEHP(1, 10 or 100 nM). The percentage of LH positive cells was quantified by flow cytometry (FC) andthe gonadotroph cell proliferation was determined by double immunostaining for LH and Ki67. ANOVA Fisher (P