Desarrollo de una metodología electroanalítica para la cuantificación de zearalenona (ZEA) en muestras de maíz

E.A. RAMIREZ; P.G. MOLINA; M.A. ZÓN; H. FERNÁNDEZ

San Jose

Congreso; XVI Congreso de la Sociedad Iberoamericana de Electroquímica, San José, Costa Rica; 2004

Abstrac The application of electroanalytical techniques to detect and quantify zearalenone (ZEA) mycotoxin that frecuently contaminates maize and foodstuff is studied in this work. Rice and maize grains were inoculated with Fusarium fungus to obtain ZEA in artificially infected samples. The electro-oxidation of ZEA adsorbed on the on the surface of glassy carbon (GC) electrodes in 20% acetonitrile (ACN) + 80% 1 M HClO4 (aqueous solution) reaction medium was studied by using square wave voltammetry (SWV). Studies were conducted to find most favorable accumulation potencial (Eacc) and accumulation time (tacc) to perform the ZEA preconcentration on the electrode surface. It was found that Eacc was any value in the range from 0.00-0.90 V and the best tacc was 120 s, respectively, for ZEA separated from extracting solution by TLC (ZEATLC) while Eacc = 0,90 V corresponded to ZEA in non separated matrix solution (ZEAmatrix). The ZEA quantitative determination was performed by SWV combined with the standard addition method. Linear plots were obtained from the net peak current (Ip.n) vs c*ZEA in the concentration range from 20 to 3184 ppb: Detection limit of 30 ppb at a signal to noise ratio of 3:1 was obtained. On the other hand, recovery experiments were performed on uncontaminated maize samples spiked with ZEA.