INVESTIGADORES
RAMIREZ Eduardo Alejandro
congresos y reuniones científicas
Título:
Studying the binding kinetics of an antigen- antibody model system by Surface Plasmon Resonance: towards the development of a biosensor
Autor/es:
C.Y. CHAIN; M. A.DAZA MILLONE; EDUARDO A. RAMIREZ; M. RUMBO; G.H. DOCENA
Lugar:
Ciudad Autónoma de Buenos Aires
Reunión:
Encuentro; 15th International Meeting on Chemical Sensors; 2014
Institución organizadora:
Centro de Convenciones de la Universidad Católica Argentina (UCA
Resumen:
Studying the binding kinetics of an antigen- antibody model system by Surface Plasmon Resonance: towards the development of a biosensor C.Y.Chain1, M.A.Daza Millone1,*, E.A.Ramirez1, M. Rumbo2, G.H. Docena2, M.E.Vela1 1 INIFTA (CONICET-UNLP) CC16 Suc4 /1900) La Plata, ARGENTINA 2 IIFP (CONICET-UNLP), La Plata, ARGENTINA. *e-mail: dazamillone@inifta.unlp.edu.ar Summary We have studied the casein-anti-casein binding kinetics using two different strategies in an SPR commercial device: i) casein was immobilized onto the solid phase and assessed with a mouse monoclonal antibody specific to bovine casein at different concentrations in solution and ii) the solid substrate was coated with the anti-casein monoclonal antibody and the binding to different concentrations of soluble casein was assessed.
