Development of an Amperometric Biosensor based on Peroxidases from Brassicas napus for the Determination of Ochratoxin A content in Peanut samples

E.A. RAMIREZ; A.M. GRANERO; M.A. ZÓN; H. FERNÁNDEZ

Journal of Biosensors and Bioelectronics

OMICS GROUP

Año: 2011 vol. S3 p. 1 - 6

2155-6210

An amperometric biosensor based on Brassica napus hairy roots peroxidases to determine ochratoxin A is proposed by the first time. The enzymatic reaction rate was studied under different experimental conditions and the kinetics parameters were determined. The method employs a dialysis membrane covered, peroxidases entrapped and ferrocene-embedded carbon paste electrode (PBHR-Fc-CP) and is based on the fact that the decreased amount of H2O2 produced by the action of PBHR is proportional to the oxidised amount of ochratoxin A in the solution. The PBHRFc- CP biosensors showed a very good stability during at least five days. The reproducibility and the repeatability were 7.7% and 5.4%, respectively, showing a good biosensor performance. The calibration curve was linear in the ochratoxin A concentration range from 1 x 10-8 to 1,4 x 10-4 mol L-1. The lowest concentration value measured experimentally for a signal to noise ratio of 3:1 was 5.7 x 10-9 mol L-1