Preliminary studies on the performance of a Hypothermic Machine Perfusion Solution (HMP) in cold static preservation (CS) of rat livers for transplant.

CARNEVALE M.E.; LAUSADA N.; GUIBERT E.E.; STRINGA P.; GAMBARO S.; RUMBO M.; GONDOLESI G.; RODRIGUEZ J.V.

Rosario

Congreso; 8° Congresso Latino Americano de Orgãos Artificiais e Biomateriais; 2014

Sociedade Latino Americana de Biomateriais, Engenharia de Tecidos e Orgãos Artificiais

The present study was undertaken to evaluate the potential use of BES-Gluconate-Polyethylene Glycol solution (BGP-HMP), originally developed for HMP with good results as those reported (1), in static cold storage of rat livers for transplants. Experimental procedures: there were in vitro and in vivo studies. The aim of the In vitro studies was to test the effect of 24 hours of static cold storage (5C) on the liver function during 90 min of reperfusion at 37 C in an ex-vivo system, and the aim of In vivo studies was to evaluate the effects of static cold storage (5C) on livers preserved 24 hours and then transplanted in recipient rats. a) In vitro studies: Male adult Sprague Dowley rats (250-300 g) were hepatectomized by a widespread surgical technique (2). Livers were preserved in BGP up to 24 hs at 5°C (n=3), after that, they were evaluated in an ex vivo perfusion system and their results were compared with fresh control livers, which were immediately reperfused in the ex-vivo system (n=3). The portal pressure and flow rate were measured and the intrahepatic resistance (IR) were calculated. Perfusate pH oscillations and enzymes (LDH, AST, and ALT) activities were evaluated during normothermic reperfusion. Also, the O2 consumption, glucose, urea production and bile flow were measured during the normothermic reperfusion period. b) In vivo studies: Livers (n=6) were obtained and preserved as was described in In Vitro studies. After 24 hours of cold storage they were transplanted (3) in receptor rats. After 30 min of reperfusion, blood samples were taken, the liver resected and biopsies also were taken for histological studies. Plasma enzymes and metabolites were assayed (AST, ALT, LDH, Bilirrubin and Creatinine). Results: a) In vitro studies - Liver function and viability during normothermic reperfusion: There were no statistical differences in portal flow and IR between groups. Portal flow was 2.54 ± 0.21 vs. 2.38 ± 0.27 (mL/min.gliver) and IR was 2.80 ± 0.39 vs. 3.27 ± 0.40 (mmHg.min.gliver/mL) with BGP-HMP and Controls respectively. The enzyme release after 90 min of reperfusion showed statistical differences (p