Hypothermic Machine Perfusion versus Cold Storage on the rescuing of livers from non-heart beating donor rats.

MATÍAS E. CARNEVALE; CECILIA L. BALABAN; EDGARDO E. GUIBERT; HEBE BOTTAI; JOAQUIN V. RODRIGUEZ

ARTIFICIAL ORGANS

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Painesville, OH; Año: 2013

0160-564X

The aim of this work was to compare the preservation efficiency of grafts excised from Non Heart Beating Donors that have suffered 45 min of warm ischemia, employing Cold Storage (CS) and Hypothermic Machine Perfusion (HMP) methods. We have developed a BES-Gluconate-Polyethylene Glycol based solution (BGP-HMP), a new solution for HMP of livers for transplants. After 24 h of HMP or CS, these livers were reperfused at 37°C with Krebs-Henseleit Dextran solution. In both procedures, portal pressure and flow were measured and the intrahepatic resistance (IR) was calculated. Perfusate pH oscillations and enzymes (LDH, AST, and ALT) activities in perfusate were evaluated during normothermic reperfusion. Also, the O2 consumption of the liver, glycogen production and bile flow were measured during the normothermic reperfusion period. Results: the portal flow (PF) and IR in both groups (n=5) showed statistical differences (p