IDENTIFICATION OF BIOMARKERS IN Salvinia minima PLANTS EXPOSED TO CHROME HEVALENT

TABERNERO ROMINA; PRADO CAROLINA DEL VALLE; CHOCOBAR PONCE SILVANA; ROSA MARIANA DANIELA

Buenos Aires

Congreso; XXXIII Argentinian meeting of plants physiologyc; 2021

Sociedad Argentina de Fisiología Vegetal

Water resources contamination of by Cr (VI) is a serious problem due to its environmental persistence, bioaccumulation in tissues and biomagnification along trophic chains. Cr (VI) is used in several industrial processes and reaches water bodies through the discharge of effluents, whose treatment is essential to reduce their impact and facilitate the rehabilitation of contaminated environments. Phytoremediation is an economical option to be implemented as part of these treatments. Exposure of plants to heavy metals triggers metabolic and physiological responses, which can be used as biomarkers during pollutants induced stress. Thus, the objective of the present work was to identify metabolites that can be used as early or late alarm signals in the presence of the contaminant. For this, S. minina plants were grown in the presence or absence of Cr (VI) during 7 days. The contents of H2O2, MDA, soluble phenols (FS) and insoluble (FI), thiolic compounds and sucrose were determined spectrophotometrically. It was observed that in fronds, MDA was a relevant parameter as an early marker of chromium stress. On the other hand, sucrose and FI levels increased late, both in fronds and lacinias, during exposure to chromium. The H2O2 content only increased towards the end of the trial, in response to prolonged exposure to the contaminant. These results show the feasibility of using these parameters as markers in future research aimed at characterizing and optimizing the use of S. minima in phytoremediation treatments.