Antimicrobial nanoformulations based on Schinus areira essential oil

CUTRO, ANDREA C.; FERREYRA MAILLARD, ANIKE P.V.; DALMASSO, P.R.; RODRIGUEZ, SERGIO; HOLLMANN, AXEL

Rosario

Congreso; L Reunión Anual SAB; 2022

Sociedad Argentina de Biofisica

Essential oil (EO) represents an interesting antimicrobial alternative to antibiotics, however, stability and water solubility represent an issue to be considered EO for potential applications. In this sense, we developed two types of hydrosoluble formulations bases on Schinus areira EO: liposomal formulations of phosphatidylcholine (DMPC and DPPC) with encapsulated EO; and green silver nanoparticles using EO as a reducing and stabilizing biocomposite. The size and charges of formulations were characterized by dynamic light scattering and microscopy experiments. In the case of liposomal formulations, chemical composition and encapsulation efficiency were also determined. Furthermore, antimicrobial activity studies against Gram-positive and Gram-negative model bacteria were carried out for both formulations. Based on the results obtained, it was proven that it is feasible to encapsulate Schinus areira EO in multilamellar liposomes of phosphatidylcholine with high efficiency. DPPC liposomes have proved to be a better encapsulation system retaining more monoterpenes from EO and therefore present antimicrobial activity against S. aureus. On the other hand, it was possible to obtain green silver nanoparticles by using S. areira EO as a reducing and stabilizing agent, which has shown antimicrobial activity against S. aureus and E. coli at low concentrations of EO. These facts show the feasibility of obtaining hidrosoluble formulations with antimicrobial potential from a natural resource with local impact.