Entrapment of Aspergillus niger MYA 135 mycelium for recycles in biocatalysis

REGNER, ERIKA LUCÍA; SALVATIERRA, HEBE NATALIA; BAIGORÍ, MARIO DOMINGO; PERA, LICIA MARÍA

Buzios

Workshop; VII Workshop on Biocatalysis and Biotransformations; 2014

Lipases (EC 3.1.1.3) are important industrial enzymes due to their versatile applications. Lipases catalyze a variety of reactions, such as partial or complete hydrolysis of triacylglycerols and reactions of esterification,transesterification and interesterification of lipids. Despite the great interests in the application of lipases in various industries, their high costs of production often restrict their use as biocatalysts. Several studies have reported the utilization of microorganisms such as bacteria, yeast and fungi as whole-cell biocatalysts in attempts to improve the cost-effectiveness of the bioconversion processes. Among the established whole-cell biocatalyst systems,filamentous fungi have arisen as the most robust whole-cell biocatalyst for industrial applications [1, 2].Entrapment in alginate beads is a technique that is widely used to improve operational stability and easy manipulation of biocatalyst applied in industrial processes [3, 4].The aim of this work was to analyze the feasibility of reuse of the entrapped Aspergillus niger MYA 135 mycelium-bound lipase (Mb) in alginate beads. The beads components were evaluated through the Plackett?Burman (PB)statistical experimental design as screening experiments tool [5].