Alteration in glucose metabolism and adipose tissue induced by zinc restriction and high fat diet during growth

ARRANZ, CRISTINA; CIANCIO DEL GIUDICE, NICOLÁS ESTEBAN; MENDES GARRIDO ABREGÚ, FACUNDO; SARAVIA FILECCIA, MELISA; MARTÍNEZ TAMBELLA, JOAQUÍN; ZAGO, VALERIA; CANIFFI, CAROLINA; SCHREIER, LAURA; ELESGARAY, ROSANA; TOMAT, ANALÍA

La Habana

Congreso; 2nd Panamerican Congress of Physiological Sciences; 2019

Sociedad Cubana de Ciencias Fisiológicas

Introduction: Metabolic diseases as obesity and diabetes lead to an increase in cardiovascular risk, morbidity and mortality. Zinc is an essential micronutrient for carbohydrates and lipids metabolism. In previous studies, we have demonstrated that zinc deficiency induces cardiovascular and renal alterations associated with an increase in blood pressure. Objective: To evaluate in adult male rats if prenatal and postnatal zinc restriction aggravates the effects of a high-fat diet (HF) during post-weaning growth in systolic blood pressure (SBP), morphology and oxidative stress of retroperitoneal adipose tissue (RPAT) and glucose metabolism. Methods: Female Wistar rats received control (C, 30ppm) or low-zinc diet (L, 8ppm) during pregnancy and lactation. At weaning (day 21) C pups were fed C (Cc) or C-HF diet (CcHF). L offspring received L (Ll) or L-HF diet (LlHF). 60% of total calories of HF hypercaloric diets correspond to fat. Oral glucose tolerance test (OGTT) was performed at day 74 (area under curve (AUC)). On day 81 SBP was evaluated, then rats were sacrificed to evaluate RPAT weight, morphology and oxidative stress (lipid peroxidation (TBARS), Catalase (CAT), superoxide dismutase activity (SOD)). Results: Table. Two way ANOVA, test Bonferroni (n=6/group *p