Fetal zinc deficiency: influence on renal nitric oxide and oxidative stress

ARRANZ CRISTINA; AGUIRRE SOFÍA; SAVIGNANO LUCÍA; CARDELLI ALCALDE DANIELA; MENDES FACUNDO; MAC LAUGHLIN MYRIAM; ELESGARAY ROSANA; CANIFFI CAROLINA; COSTA MARÍA DE LOS ÁNGELES; TOMAT ANALÍA

Milán

Congreso; 21st European Meeting of Hypertension.; 2011

European Society of Hypertension.

Moderate and marginal zinc deficiency during pregnancy is a nutritional insult to fetal and postnatal developmen have demonstrated that moderate zinc deficiency in rats during intrauterine and postnatal growth is also a model fetal programming of hypertension and renal diseases in adult life. At weaning, fetal and lactation zinc deficienc induced a reduction in glomeruli number and a compensatory hypertrophy in the remaining nephrons. Objective: To evaluate renal nitric oxide system and oxidative stress at 21 days of life and to determine if there a differences in response to fetal and lactation zinc deficiency. Methods: Wistar rats were exposed from the beginning of pregnancy up to adulthood: low (L, 8 ppm) or control(ppm) zinc diet. At day 21, male (m) and female (f) offspring of each group of mothers (Cm, Lm, Cf and Lf) were sacrificed to determine renal nitric oxide synthase (NOS) activity with L-(U14C)-arginine (pmol/min×g tissue)and eNOS and iNOS protein abundance by western blot (% density/β actin). Renal oxidative stress was evaluated measuring thiobarbituric acid-reactive substances (TBARS), glutathione concentration (GLUT), superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase activity (GPx). Results: NOS activity was decreased in Bm (237±5*) and Bf (245±7‡) compared with Cm (302±5) and Cf (317 respectively. eNOS content was higher in Bm (43±2*) than Cm (30±2), but in Bf (42±4) and Cf (36±1) was similar iNOS content showed no differences between groups (Cm: 32±4; Bm: 39±1; Cf: 39±3 and Bf: 32±3) *p