Exposure to high fat diet early in life: evaluation of cardiometabolic outcomes in male offspring

SORIA, DAMIÁN ARIEL; ZAOBORNYJ, TAMARA; OVIEDO, MELANIE; RAMÍREZ, HERNÁN; TOMAT, ANALÍA; ARRANZ, CRISTINA; ELESGARAY, ROSANA; ROMERO, MARIANA; CANIFFI, CAROLINA

La Plata

Congreso; ISHR-Lat Section Meeting; 2022

International Society for Heart Research Lat-Section

Introduction: The intake of dietary fat is increasing, and the incidence of overweight pregnant women as a result of a maternal high-fat diet (HFD) is thus increasing worldwide. The intrauterine environment associated with a HFD increases the risks of poor metabolic health and non-communicable diseases laterin life.Objetive: To evaluate the effects of maternal HFD on metabolic homeostasis and cardiac oxygen consumption in offspring.Experimental design: Wistar female rats fed either HFD (60% calories from fat) or standard diet (SD) from pregnancy to offspring weaning. At 21 days of life, offspring were weighted (body weight, BW) and fasting glycemia was determined using a glucometer in male (m) offspring. Finally, male offspring were sacrificed. Retroperitoneal adipose tissue (rAT) and cardiac left ventricle (LV) were removed and weighted. Also, tibial length (TL, cm) was measured. In LV, oxygen consumption was measured, and the respiratory control (RC) was calculated as the ratio between state 3/state 4 respiration rates. The protocol was approved by Ethic Committee CICUAL-FFyB-UBA. Results are expressed as mean±SEM.Statistical analysis: Student test (n=7-8 rats/group; *p