Renal oxidative stress in hypertensive rats. Atrial natriuretic peptide chronic treatment.

SAVIGNANO LUCÍA; MOLINARI AGUSTINA; BOUCHET GONZALO; AGUIRRE SOFÍA; CANIFFI CAROLINA; ROMERO MARIANA; ELESGARAY ROSANA; ARRANZ CRISTINA; COSTA MARÍA DE LOS ÁNGELES

Buenos Aires

Workshop; International Workshop in oxidative stress and antioxidants.; 2009

Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires.

The aim of the study was to investigate the effects of chronic infusion with atrial natriuretic peptide (ANP) on renal oxidative stress in spontaneously hypertensive rats (SHR). Methods: 8 weeks-old SHR and Wistar Kyoto (WKY normotensive rats) were infused (14 days, subcutaneous osmotic pumps) with ANP (100 ng/hr/rat) or saline (S). After experimental period, we determined in kidney: thiobarbituric acid-reactive substances (TBARS, nmol/mg protein), glutathione concentration (mg/mg protein), and activity of: glutathione peroxidase (GPx, pmol/min.mg protein), catalase (CAT, pmol/mg protein) and superoxide dismutase (SOD, USOD/mg protein). Results: TBARS Glutathione GPx CAT SOD WKY S 0,113±0,015 0,271±0,038 1,449±0,230 0,847±0,102 7,691±0,505 WKY ANP 0,098±0,010 0,398±0,028* 1,890±0,302 0,956±0,098 8,021±1,654 SHR S 0,162±0,025* 0,178±0,046* 3,315±0,225* 0,951±0,105 7,098±0,653 SHR ANP 0,082±0,009# 0,374±0,027# 3,920±0,845 1,101±0,142 8,452±0,779 *p< 0,01 vs WKY S; #p