Glial response to nutrient deprivation in experimental models of Alzheimer?s disease.

AMAL GREGOSA|; POMILIO CARLOS; ÁNGELES VINUESA; JESSICA PRESA; FLAVIA SARAVIA; JUAN BEAUQUIS

Congreso; Reunión anual de la Sociedad Argentina de Investigaciones Clínicas; 2018

Alzheimer?s disease is a neurodegenerative pathology clinically characterized by progressive and irreversible cognitive decline. Dietary restriction (DR) has been proposed as a possible neuroprotective strategy able to increase neurotrophic factors levels, enhance life span, promote autophagy and reduce oxidative stress. Our previous results demonstrated that transgenic animals modelling Alzheimer?s disease and exposed to DR reverted cognitive impairment, showed a reduction of amyloid beta plaque load in the hippocampus and presented an increase in hippocampal neurogenesis. Our present objective was to evaluate the glial response to nutrient restriction in adult PDAPP-J20 transgenic (Tg) mice and in an in vitro approach. The in vivo experiment consisted in a periodic DR protocol alternating ad libitum periods (9 days) with DR periods (5 days, 60% daily intake) for 1.5 months, from 6.5 to 8 months of age. We found that microglial soma size, morphological parameter compatible with cell activation, was increased in Tg mice (p