Type 1 IGF Receptor Localization in Paediatric Gliomas: Significant Association with WHO Grading and Clinical Outcome

CLÉMENT, FLORENCIA* EQUAL CONTRIBUTION; MARTIN, AYELEN* EQUAL CONTRIBUTION; VENARA, MARCELA; DE LUJÁN CALCAGNO, MARIA; MATHÓ, CECILIA; MAGLIO, SILVANA; LOMBARDI, MERCEDES GARCÍA; BERGADÁ, IGNACIO; PENNISI, PATRICIA A

Hormones and Cancer

Springer

Año: 2018

1868-8497

Nuclear localization of insulin-like growth factor receptor type 1 (IGF-1R) has been described as adverse prognostic factor insome cancers.We studied the expression and localization of IGF-1R in paediatric patients with gliomas, as well as its associationwith World Health Organization (WHO) grading and survival. We conducted a single cohort, prospective study of paediatricpatients with gliomas. Samples were taken at the time of the initial surgery; IGF-1R expression and localization were characterizedby immunohistochemistry (IHC), subcellular fractionation and western blotting. Tumours (47/53) showed positive stainingfor IGF-1R by IHC. IGF-1R nuclear labelling was observed in 10/47 cases. IGF-1R staining was mostly non-nuclear in lowgradetumours, while IGF-1R nuclear labelling was predominant in high-grade gliomas (p = 0.0001). Survival was significantlylonger in patients with gliomas having non-nuclear IGF-1R localization than in patients with nuclear IGF-1R tumours (p =0.016). In gliomas, IGF-1R nuclear localization was significantly associated with both high-grade tumours and increased risk ofdeath. Based on a prospective design, we provide evidence of a potential usefulness of intracellular localization of IGF-1R asprognostic factor in paediatric patients with gliomas.Florencia Clément and Ayelen Martin contributed equally to this work.