Light-induced stress triggers the production of phytosterols in Haematococcus pluvialis

SCODELARO BILBAO PAOLA; DAMIANI, MARIA CECILIA; DELUCCHI FEDERICO; SALVADOR GABRIELA; LEONARDI PATRICIA INES

Mar del Plata

Congreso; SAIB; 2015

Light-induced stress triggers the production of phytosterols in HaematococcuspluvialisScodelaro Bilbao, P1,2,3; Damiani, C1,2; Delucchi, F1,3; Salvador, G1,3; Leonardi P1,2.1Dpto. Biol., Bioq y Farm.-UNS, 2CERZOS, 3INIBIBB, 4PLAPIQUI -CONICET.Bahía Blanca, Argentina.pscodela@criba.edu.arHaematococcus pluvialis is an oleaginous microalga proposed as a source otriacylglycerol (TAG) for biodiesel production. The aim of this work was to describte the effect of light-induced stress on lipid accumulation in a native e . For this end, H. pluvialis CCALA 1081 from Bahía Blanca (Arg.) was grown under controlconditions or light-induced stress (300μmol m-2 s-1) for 3, 6 and 12 days. Then, thelipid profile was analyzed in order to determine TAG and phytosterol (PHS)contents, the fatty acid profile of TAG and the composition of the PHS fraction.After 3 days of light-induced stress, the levels of both TAG and PHS significantlyincreased over each control (210% and 1251%, respectively). Palmitic, oleic,linoleic and α linolenic fatty acids were increased whereas caproic acid contentdiminished in H. pluvialis under stress. Light-induced stress also reduced β-sitosterol content while clerosterol, brassicasterol and Δ7-campesterol augmentedin the PHS fraction. No significant levels of lipid peroxidation were detected afterlight-induced stress. Finally, PHS extracts obtained after light-induced stressshowed increased antioxidant capacity when compared with control conditions.These results altogether suggest that high light stress induces theaccumulation ofPHS with antioxidant properties in H. pluvialis withpotential therapeutic and industrial applications.