Multiapproach for repositioning drugs for colorectal cancer treatment

ANSELMINO, LUCIANO; BAGLIONI, MARÍA VIRGINIA; FLORENCIA MALIZIA; MAURICIO MENACHO MÁRQUEZ

Mar del Plata

Congreso; Reunión Anual SAIC-SAFE-SAB-SAP 2019; 2019

SAIC

Drug repositioning in oncology refers to the use of drugs indicated for the treatment of certain pathologies that have proved some anticancer effects. There are two main ways to select drugs for repositioning: "text mining-based" and "in silico-based" approaches. The first one uses prior knowledge of the mode of action of a drug, while the second one detects putative target genes from genetic expression data using computational tools to propose an active drug. In this work we have used these two approaches to select a group of candidates drugs to reposition for colorectal cancer (CRC) treatment. First, by MTT-based proliferation assays we evaluated the effect of a group of drugs, either individually or in a combined manner on HCT116 and HT29 cell lines. Form all the combination tested, we focused on Metformin (M; diabetes treatment) and propranolol (P; hypertension treatment) combination because it showed a strong growth inhibition even combining low doses of both drugs (P