Rac1-DEPENDENT Vav2 INVOLVEMENT IN MELANOMA

MACARENA MAMBERTO; NAHUEL CESATTI LALUCE; MAURICIO MENACHO MÁRQUEZ

Congreso; Reunión Conjunta SAIC. SAI. AAFE. NANOMED-AR; 2021

Melanoma is the most dangerous form of skin cancer, associatedwith an increasing incidence in the population.Vav proteins are guanosine nucleotide exchange factors (GEFs)for the Rho GTPase family (mainly composed by Rac1, RhoA andCdc42). In previous works we explored the role of Vav2 in eventsassociated to melanoma development. We modulated Vav2 expression in the mouse-derived melanoma cell line B16-F0 observing thatVav2 is involved in cellular processes linked to proliferation, migration and cytoskeletal rearrangement, promoting melanoma development.In this work, we studied the dependency of these phenotypes onRac1 introducing by lipotransfection a fast cycling form of Rac1(Rac1F28L) in Vav2-deficient cells. By MTT based methods we observed that defective proliferation and migration associated to reduced levels of Vav2 (P