EXPLORING THE ROLE OF PARKINSON DISEASE-RELATED PROTEINS IN PROSTATE CANCER

EUGENIA CRESPO; FLORENCIA MALIZIA; MAURICIO MENACHO MÁRQUEZ

Mar del Plata

Congreso; REUNIÓN ANUAL DE SOCIEDADES DE BIOCIENCIA 2019; 2019

Synucleins are small proteins expressed primarily in neural tissue and in certain tumors. Alpha-synuclein (aS) was recently connected to melanoma development and gamma-synuclein (gS) was associated to a wide range of cancer types. Both proteins are implicated in the development of Parkinson disease (PD). Interestingly, recent studies have shown a direct association between PD and the development of melanoma and prostate cancer. Our goal in this work was to explore the putative role aS and gS could have in the development of prostate cancer. We analyzed the expression of aS and gS in human prostate cancer cell lines (PC3, DU145 and LNCaP). By Western blot (WB) and immunocytochemistry we observed that both proteins were expressed in these cell lines, and by differential lysis experiments with detergents we determined that aS was preferentially present at the cytosol, while gS was detected mainly at the membrane fraction of PC3 cells. By standard shRNA techniques and the use of expression vectors, we modulated aS and gS expression in PC3 cells (p0.05). Nevertheless, by wound healing assays, we observed that PC3 cells overexpressing gS showed a more migratory behaviour than control cells (p