ω-6 AND ω-3 FATTY-ACIDS ON EARLY STAGES OF MICE SUBMANDIBULAR GLANDS TUMOR

SCHERMA ME; G.M. MAZZUDULI; SILVA RA

Congreso; LII Reunión Anual de la Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2016

Scherma ME1; Madzzudulli G2; Silva RA2; Repossi G2; Brunotto M1; Pasqualini ME2 1Fac. Odontología-UNC; 2 Fac. Cs. Médicas-(INICSA-CONICET-UNC)E-mail: eugescherma@hotmail.com The aim of this work was to assess the impact of diets enriched in PUFAs ω-3 and ω-6 on the lipid profile of submandibular glands (SMG) cell membrane and their effect on cell cycle regulation and apoptosis, evaluated by TP53 and Ki-67 expression in 9,10- dimethyl-1,2-benzanthracene (DMBA) induced tumor development in SMG in a murine model.To generate tumorigenic changes, SMG mice of the experimental group were injected with 50μl of 0.5% of DMBA. Both control (no DMBA) and experimental groups of BALB/c mice were fed with: chia oil (ChO),rich in ω-3 fatty acid or corn oil (CO), rich in ω-6/ω-3 fatty acid; or safflower (SO) oil, rich in ω-6 fatty acid. The PUFAs tumor cell profile was analyzed by gas chromatography and eicosanoids by HPLC. Apoptosis was measured by TUNEL and TP53/Ki-67 were inmmunomarked. Arachidonic acid increased significantly in animals treated with DMBA and fed with SO and CO diet. EPA and ALA increased significantly in animals fed with ChO in control and treated with DMBA. Eicosanoids showed significant variations between animals treated with DMBA and their respective controls. Results demonstrated novel differential effects of ω-3 and ω-6 PUFAs on the regulation of early tumorigenesis events in murine SMG injected with DMBA. This knowledge may help to develop chemoprotective treatments, therapeutic agents and health promotion and prevention activities in humans