INVESTIGADORES
FRANCO Diana Lorena
congresos y reuniones científicas
Título:
ENA/VASP downregulation triggers cell death by impairing axonal maintenance in hippocampal neurons
Autor/es:
D.LORENA FRANCO , CAROLINA REZAVAL , ALFREDO CÁCERES , ALEJANDRO F. SCHINDER AND M. FERNANDA CERIANI
Lugar:
Huerta Grande, Cordoba
Reunión:
Congreso; Primera Reunion Conjunta de Neurociencias; 2009
Institución organizadora:
Abstract book
Resumen:
Neurodegenerative diseases encompass a broad variety of motor and cognitive disorders that are accompanied by death of specific neuronal populations or brain regions. Cellular and molecular mechanisms underlying these complex disorders remain largely unknown. In a previous work we searched for novel Drosophila genes relevant for neurodegeneration and singled out enabled (ena), which encodes a protein involved in cytoskeleton remodelling. To extend our understanding on the mechanisms of ENA-triggered degeneration we now investigated the effect of silencing ena ortholog genes in mouse hippocampal neurons. We found that ENA/VASP downregulation led to neurite retraction and concomitant neuronal cell death through an apoptotic pathway. Remarkably, this retraction initially affected the axonal structure, showing no effect on dendrites. Reduction in ENA/VASP levels blocked the neuritogenic effect of a specific RhoA kinase (ROCK) inhibitor, thus suggesting that these proteins could participate in the Rho-signaling pathway.