PROTECTIVE ACTIONS OF GENISTEIN IN A MODEL OF HYPOESTROGENISM AND OBESITY

CEPEDA, S.; CUTINI, P.; VALLE, MI; CAMPELO, AE.; SANDOVAL MJ; MASSHEIMER, VL.

Mar del Plata

Congreso; REUNION ANUAL DE SOCIEDADES DE BIOCIENCIAS; 2023

SAIC

Menopause is associated with higher prevalence of chronic diseases such as obesity and osteoporosis. Genistein (Gen) has beenproposed as a natural therapy to counteract these diseases. Previously in our lab we demonstrated that, in the absence of ovarianfunction, obesity exhibited a deleterious action on bone metabolismthrough an enhancement in bone oxidative stress and reducing mineralization. In this work, we studied the effect of Gen on bone stressinduced by obesity and hypoestrogenism. Females Wistar ratswere bilaterally ovariectomized (OVX) and fed with a high-fat diet(OVX-Ob) for 10 weeks. Experimental systems: diaphysis of femurs(DF) and retroperitoneal AT explants isolated from OVX-Ob rats, orprimary culture of DF-osteoblast (OBL). DF, AT, OBL were in vitroexposed to Gen for different interval of treatment. In DF, Gen (18 h)reduced H2O2 (DC fluorescent probe) levels and stimulated VEGF(ELISA kit) synthesis respect to control (21.6±0.8vs16.9±1.1μmol/gtissue, p