Desarrollo y evaluación de formulaciones herbáceas con actividad antibacteriana a base de Sepigel

PÉREZ ZAMORA, CRISTINA; MORETTÓN, MARCELA; CYNTHIA SANCHEZ; NUÑEZ, MARÍA BEATRIZ; CHIAPPETA, DIEGO

Rosario

Congreso; Reunión Internacional de Ciencias Farmacéuticas; 2016

Universidad Nacional de Córdoba

Sepigel® is a liquid dispersion of polyacrylamide in isoparaffin. It demonstrates affinity for both, hydrophilic and oleophilic substances and it is usually employed in cosmetic development. It also acts as a stabilizer agent and it can be used to thicken oil/water emulsions and gels.The aim of the present work was the development of topic Sepigel® formulations employing a combination of two dried herbal extracts with antimicrobial properties, Lippia alba and Lippia turbinate. Gels composition was optimize to get i) suitable characteristics for applied on skin and ii) an adequate physicochemical stability over time at room temperature (1 year).Briefly, gels were prepared by simple dispersion of Sepigel® (2, 4, 6 and 8% w/w) in distilled water under mechanic stirring (7000 rpm) at room temperature over 30 minutes. Then, the herbal extracts, etanol and, propylene glycol were added and the mechanic stirring was continued over 1 h, until a homogenous gel was obtained. Herbal extracts-free gels were also prepared as controls. Gels were characterized by i) organoleptic characteristics, ii) pH, iii) homogeneity, iv) viscosity, v) total phenols content, vi) conductivity and vii) antibacterial activity over 1 year. Also a microbiological control was performed according to Argentina Pharmacopeia seventh edition.Initially (day 0), herbal gels showed brown color, characteristic odor of herbal species and a pH value of 5.5 The total phenols content was 4.15 ± 0.12 mg gallic acid equivalent/g. After the incorporation of the herbal extracts into the gels, their viscosity was decreased and their conductivity was increased respects to the control gels. Further, herbal gels demonstrated antibacterial activity against three different Staphylococcus strains, where inhibition halos (10-12 mm) were observed.Moreover, gels demonstrated no change on their antibacterial activity over 1 year stored at room temperature, and variations in other evaluated properties were within an acceptable range.