Suppression of gamma delta T cell function by neutrophils

SABBIONE, FLORENCIA; GABELLONI, MARÍA LAURA; ERNST, GLENDA; GORI, SOLEDAD; TREVANI, ANALIA; GEFFNER, JORGE; JANCIC, CAROLINA

EUROPEAN JOURNAL OF IMMUNOLOGY

WILEY-V C H VERLAG GMBH

Lugar: Weinheim; Año: 2013

0014-2980

gamma delta T cells have been shown to stimulate the recruitment and activation of neutrophils through the release of a range of cytokines and chemokines. Here, we investigated the reverse relationship, showing that human neutrophils suppress the function of human blood gamma delta T cells. We show that the upregulation of CD25 and CD69 expression, the production of IFN-gamma, and the proliferation of gamma delta T cells induced by HMBPP are inhibited by neutrophils. Spontaneous activation of gamma delta T cells in culture is also suppressed by neutrophils. We show that inhibitors of prostaglandin E2 and arginase I do not exert any effect, although in contrast, catalase prevents the suppression of gamma delta T cells induced by neutrophils, suggesting the participation of neutrophil-derived ROS. We also show that the ROS-generating system xanthine/xanthine oxidase suppresses gamma delta T cells in a similar fashion to neutrophils, while neutrophils from chronic granulomatous disease patients only weakly inhibit gamma delta T cells. Our results reveal a bidirectional cross-talk between gamma delta T cells and neutrophils: while gamma delta T cells promote the recruitment and the activation of neutrophils to fight invading pathogens, neutrophils in turn suppress the activation of gamma delta T cells to contribute to the resolution of inflammation.