Desing and characterization of liposomes containin stimuli-responsive molecules

SOTO ESPINOSA SL; CARBALLO R.; FLOR S; REZZANO I

Tucuman-Tucuman

Encuentro; XLV Reunión anual de la Sociedad Argentina de Biofísica; 2016

Sociedad Argentina de Biofísica

We have design stable redox and optical active liposomes where the optical moiety was a metalloporphyrin and the redox part was ferrocene. Through the combination of these stimuli-responsive molecules and phospholipid assemblies, local control over the payload release based on an endogenous or exogenous trigger confers a spatial and temporal specificity [1]. We studied two different modified molecules (ferrocene and protoporphyrin IX esterified with octadecylamine, namely FC-ODA and PPIX-ODA) and compare their performance with unmodified molecules [2]. Liposomes were synthesized as describes in [3]. A solution of PPIX and PPIX-ODA 3.3 µM were added to the liposome containing samples (in ratio 1:5) and were mixed on a shaker for 6 h to reach the full binding equilibrium. The Fc and Fc-ODA were added during liposome synthesis. In the present work we analysed the behaviour of liposomes binding of different molecules. The response was measured by electrochemical and fluorescence signal, electrophoretic mobility and UV spectroscopy. Different behaviours were found for different systems evaluated.