Bacterial Cellulose Hydrogel Loaded with Lipid Nanoparticles for Localized Cancer Treatment

CACICEDO, M.L.; ISLAN, G.A.; LEÓN, I.E.; ÁLVAREZ, V.A.; CHOURPA, I.; ALLARD-VANNIER, E.; GARCÍA-ARANDA, N.; DÍAZ-RIASCOS, Z.V.; FERNÁNDEZ, Y.; SCHWARTZ, S.; ABASOLO, I.; CASTRO, G.R.

COLLOIDS AND SURFACES B-BIOINTERFACES

ELSEVIER SCIENCE BV

Lugar: Amsterdam; Año: 2018

0927-7765

The use of hybrid materials, where a matrix sustains nanoparticles controlling the release of the ACCEPTED MANUSCRIPTchemotherapeutic drug, could be beneficial for the treatment of primary tumors prior or aftersurgery. This localized chemotherapy would guarantee high drug concentrations at the tumor sitewhile precluding systemic drug exposure minimizing undesirable side effects. We combined bacterial cellulose hydrogel (BC) and nanostructured lipid carriers (NLCs)including doxorubicin (Dox) as a drug model. NLCs loaded with cationic Dox (NLCs-H) orneutral Dox (NLCs-N) were fully characterized and their cell internalization and cytotoxicefficacy were evaluated in vitro against MDA-MB-231 cells. Thereafter, a fixed combination ofNLCs-H and NLCs-N loaded into BC (BC-NLCs-NH) was assayed in vivo into an orthotopicbreast cancer mouse model. NLCs-H showed low encapsulation efficiency (48%) and fast release of the drug while NLCs-Nshowed higher encapsulation (97%) and sustained drug release. Both NLCs internalized viaendocytic pathway, while allowing a sustained release of the Dox, which in turn rendered IC50values below of those of free Dox. Taking advantage of the differential drug release, a mixtureof NLCs-N and NLCs-H was encapsulated into BC matrix (BC-NLCs-NH) and assayed in vivo,showing a significant reduction of tumor growth, metastasis incidence and local drug toxicities.