Anticancer and antimetastatic activity on A549 cell line of a new compound

JULIA MERMOT; JAVIER L. RODRÍGUEZ; JUAN J. MARTÍNEZ MEDINA; LUCIANA G. NASO

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

Sociedad Argentina de Investigación Bioquímica y Biología Molecular

Many flavonoids are natural chelators and flavonoid-metal complexes have shown to possess significant cytotoxic activity. Chrysin is a flavone distributed in plants which was reported to have many biological activities. In this study, a complex of chrysin with magnesium(II) (Mgchrys) was synthesized and its anticancer and antimetastatic activity on A549 cell line were determined. The IC50 values of chrysin were determined to be >100, 66.4±4.2 and 37.3±3,3 µM at 24, 48 and 72 h, respectively. Mgchrys improved the action of the free ligand being its IC50 values 83.9±2.9, 28.2±3.5 and 21.1±3,2 µM at the same time. Ligand and complex did not behave like cytotoxic agents in the normal cell line. Oxidative stress is the probable mechanism of action because the compounds increased levels of ROS. Some morphological changes (increment of cytoplasm condensation, presence of pycnotic nuclei) were observed when the cells were incubated with Mgchrys. The adhesion to fibronectin ability of A549 cells treated with Mgchrys decreased 40 % in comparison with the control. Chrysin and MgCl2 did not affect adhesion. Mgchrys showed significant inhibition of migration (45%) as compared to the ligand (16%). A 47% reduction on cell invasion was observed when cells were treated with Mgchrys. This study shows that Mgchrys could be considered as a promising chemotherapeutic agent.