Polyphenol content and antiradical activity of Euphorbia serpens extract stored in different forms and under different conditions

MATÍAS E. MOSCHEN; JUAN J. MARTÍNEZ MEDINA; CRISTINA M. PÉREZ ZAMORA; MARÍA B. NUÑEZ

Rosario

Congreso; 7ma Reunión Internacional de Ciencias Farmacéuticas; 2023

Universidad Nacional de Córdoba y Universidad Nacional de Rosario

Euphorbia species have medicinal properties due to the presence of several secondary metabolites, mainly terpenoids, flavonoids, and polyphenols. The encapsulation of the extracts is a strategy to achieve the stability of these compounds. The aim of this work was to evaluate the polyphenol content and the antiradical activity of different forms of Euphorbia serpens Kunth extract (fluid, dry, and encapsulated extract) stored under different conditions. One part was used as it was obtained (fluid extract), another part was dried to constant weight (dry extract), and a third part was used to prepare capsules with sodium alginate (1% w/v) and calcium chloride (5% w/v) by using the ionic gelation technique. Storage conditions were: 1) 40 °C and 75% relative humidity (RH); 2) 25 °C and 58% RH; 3) 8 °C and 58% RH. The extracts were analyzed to determine the content of phenolic compounds by the reaction with the Folin-Ciocalteau reagent, and their antiradical activity by using the DPPH radical at 0, 30, 90, and 180 days. There were fluctuations in the polyphenol content throughout the time, but at 180 days, the values were close to the initial value for all samples. Regarding the antiradical activity, the initial values were 72 ± 2%, 80 ± 3, and 23 ± 2%, for fluid, dry, and encapsulated extracts, respectively. Fewer fluctuations were detected throughout the time, thus increasing the antiradical activity after 180 days. These results suggest that regardless of the form of the extracts of E. serpens and the storage conditions evaluated, their antioxidant properties are preserved for at least 180 days.