STUDY OF THE ROLE OF EISOSOMAL MEMBRANE DOMAINS IN AGING

VALENTINA SALZMAN; MARIBEL PATERNOSTE; CAROLINA DO PAZO; PABLO AGUILAR

Mar del Plata

Congreso; LI Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2015

SAIB

Saccharomyces cerevisiae plasma membrane (PM) organization is partially dependent on eisosomes assembly, large protein complexes that delimit nanoscale PM invaginations. Eisosomes are composed of more than 25 proteins including transporters, signaling molecules and proteins of still unknown molecular function. Some of these proteins have been reported to be involved in the cellular aging process. We are interested in understanding eisosomes ́ role in aging using S. cerevisiae, an experimental model that has contributed to the identification of many genes involved in aging in mammals, flies and worms. In knockout strains for PIL1, a gene encoding a major eisosomal structural protein, the PM topography is abnormal with large invaginations and eisosomes ́ organization is lost. Performing both replicative and chronological yeast aging assays we have found that PIL1 deletion leads to significantly enhanced longevity in different S. cerevisiae genetic backgrounds, strongly suggesting that eisosome structure plays a key role in the yeast aging process. In order to characterize the observed pro-longevity effect, we analyzed the interaction between PIL1 and key genes involved in aging pathways. Describing eisosomes ́ role in aging will likely contribute to further understanding of PM domains? functions as well as the complex aging signaling network.