A plasma membrane E-MAP Reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking.

AGUILAR, P.S.; FRÖHLICH, F.; REHMAN, M.; SHALES, M.; ULITSKY, I.; OLIVERA-COUTO, A.; BRABERG, H.; SHAMIR, R.; WALTER, P.; MANN, M.; EJSING, C.S.; KROGAN, N.J. AND WALTHER, T.C.

NATURE STRUCTURAL & MOLECULAR BIOLOGY

NATURE PUBLISHING GROUP

Lugar: Londres; Año: 2010 vol. 17 p. 901 - 908

1545-9985

The plasma membrane delimits the cell and controls material and information exchange between itself and the environment. How different plasma-membrane processes are coordinated and how the relative abundance of plasma-membrane lipids and proteins is homeostatically maintained are not yet understood. Here, we used a quantitative genetic interaction map, or E-MAP, to functionally interrogate a set of ~400 genes involved in various aspects of plasma-membrane biology, including endocytosis, signaling, lipid metabolism and eisosome function. From this E-MAP, we derived a set of 57,799 individual interactions between genes functioning in these various processes. Using triplet genetic motif analysis, we identified a new component of the eisosome, Eis1, and linked the poorly characterized gene EMP70 to endocytic and eisosome function. Finally, we implicated Rom2,a GDP/GTP exchange factor for Rho1 and Rho2, in the regulation of sphingolipid metabolism.